Project

Part:BBa_M31513:Design

Designed by: Amirah Khan   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-26)


Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1307
    Illegal BamHI site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to consider the fact that basepairs within the regions that encoded for genes could not be altered too much since we want to maintain functionality of M13 after refactoring this segment of its genome


Source

The source for this part was the genmoic sequence of the M13 bacteriophage

References